Preparation of silica-chitosan nanoparticles encapsulating different water soluble compounds and their release profiles

• Main Authors: Yuan-Shuo Chang, 張元碩
• Other Authors: Ke-Liang B. Chang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/97519059243448011995

碩士 === 國立臺灣海洋大學 === 食品科學系 === 97 === Composite nanoparticles was prepared by silica and chitosan to form silica-chitosan nanoparticles (SCNPs) and encapsulate three water soluble components, ascorbic acid, amoxicillin, and bovine serum albumin (BSA), respectively, as model drug. Higher ascorbic acid concentrations results in more acidic solution during the preparation of nanoparticles. The lowered pH decreased the diameter, yield and polydispersity (PDI) of nanoparticles. Higher concentration of BSA would increase the nanoparticle diameter and encapsulation efficiency. SEM and TEM micrographs indicated that SCNPs was formed by the polycondensation of silicic acid into silica particles. FT-IR confirmed that binding reaction occurred between silica and chitosan, and the functional groups of nanoparticles showed no significant difference after the encapsulation of all model drugs. The zeta potential was changed after the encapsulation of different model drugs and led to different particle stability and dispersity. The antioxidative activity was determined by comparing the DPPH scavenging ability of model drug before and after encapsulation. Test results confirmed that the bioactivity of component was unchanged after the encapsulation process. Confocal microscopy images and dissolution testing at different pH environments indicated that SCNPs could enter cultured cells through endocytosis and may release encapsulated component in the cells.