| Summary: | 碩士 === 國立臺灣師範大學 === 生命科學研究所 === 97 === Gentiana L. is belonged to Gentianaceae, Gentianaeae. The Gentiana in Taiwan includes perennial and annual herbaceous species. Both Gentiana flavomaculata and G. flavomaculata var. yuanyanghuensis are annual and endemic in Taiwan. Though there are some distributional and morphological differences between these two species, however, highly similarity still exists among taxons. In order to test whether G. flavomaculata and G. flavomaculata var. yuanyanghuensis were genetically differentiated and genetic diversity characteristics, chloroplast rps4 was combined with microsatellite DNA to discuss phylogenetic structure and genetic differentiation. The result showed only 0.2 % nucleotide diversity between G. flavomaculata and G. flavomaculata var. yuanyanghuensis and eight haplotypes were found among all chloroplast segments, which were highly shared by two species according to Neighbor - Joining K2P tree. The AMOVA showed congruent result of chloroplast and microsatellite and low genetic differentiation among species (ΦCT = 0.064, cpDNA; ΦCT =0.057, microsatellite DNA ), implying genetic exchange mechanism among populations of these two specie, and these two species could not be spported as different species phylogenetically. As a result, we suggested G. flavomaculata and G. flavomaculata var. yuanyanghuensis should temporarily be treated as a infra-species based on phylogenetic evidence.
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