The study of topically applied nanonized silver gel enhances wound healing in diabetic rat skin

• Main Authors: An-Ji Chao, 趙安基
• Other Authors: Chin-En Tsai
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/00700250152034443342

碩士 === 國立屏東科技大學 === 獸醫學系所 === 97 === The purpose of this study is to investigate the effects of nanonized silver gel (nAg gel) on the delayed wound healing in diabetic rats. Adult male SD rats (BW: 375 ± 25g) were divided at random into 4 groups (A, B, C and D; six rats per group), Groups A and B, and Groups C and D were injected intraperitoneally with 0.1 mol/L citrate buffer (1 mL/kg/week), and streptozotocin (STZ, 70 mg/kg/week), respectively, for 3 weeks. On day 21 after STZ and buffer injected, the diabetic and non-diabetic control rats were anesthetized with isoflurane in created operation table. The six (1 × 1 cm2) full-thickness skin wounds in the dorsal thorax (3 on each side) in each of groups were created. Nondiabetic rats were served as controls (group A: wounds treated with gel, 50 μL/wound; group B: wounds treated with nAg gel, 50 μL/wound). Diabetic rats were divided into 2 experimental groups (group C: wounds treated with gel, 50 μL/wound; group D: wounds treated with nAg gel, 50 μL/wound). On the 0, 3rd, 5th, 7th and 14th day and 21st day, each of the wound healing areas was measured and the histopathological examination (light microscope and electric microscope) was observed. Results: 1. Poor wound healing in diabetes mellitus was characterized by decreased wound collagen content. 2. The value of blood glucose in diabetic rats was steadily higher than 300 mg/dL. 3. The body weight in diabetic rats was thinner than normal rats. 4. In nAg treated rats, the wound healing areas and re-epithelialization were greater than that in untreated rats (p＜0.05). Nanonized silver maybe eventually become a valuable adjunct for chronic wounds, including diabetic or other non-healing chronic ulcer.