| Summary: | 碩士 === 國立屏東科技大學 === 環境工程與科學系所 === 97 === Industrial development consume huge amount of coal, petroleum, natural gas and release massive amount of greenhouse gases. Couple with population growth and forests cutting, greenhouse gases cause global warming and many climate change induced disasters recently. After realized these not-natural disasters will origin large-scale ecosystem broken, human life threaten, and economy crash, The United Nations had drawn up The United Nation Framework Convention on Climate Change and Kypto Protocol to limit greenhouse gases emission. This study is also trying to do the effort on global warming issue. We like to find out the best growth condition for freshwater microalgae CO2 fixation in South Taiwan.
Chlorella vulgari collected from pound in NPUST was selected as material. The M4N medium used to supply essential nutrients for microalgae growth. Six parameters were choose to exam microalgae growth rate which were: algae injecting concentration (329 mg/L、522 mg/L、648 mg/L and 770 mg/L); temperature (15, 25 and 35℃); CO2 concentration (5, 10, 15 and 20 %); light cycles (night/day) (12 and 24 hours); pH value (5, 6, 7 and 8); and light source (plant lamp, fluorescent tubes, and LED red lamp). The culture cycle was incubated in a growth chamber for 15 days. Spectrophotometer was used to measured growth curve at wavelength 680nm.
In summary, the maximum biomass and CO2 fixation rate was at the condition that solution pH = 7.0, 24 hours lighting by fluorescent tubes, 10% CO2 injected, 35℃, and the initial Chlorella vulgaris concentration was 522mg / L. Under these conditions we could get 4,659 mg/L of microalgae biomass and 5,594 mgCO2/L of CO2 fixation.
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