| Summary: | 碩士 === 國立屏東科技大學 === 畜產系所 === 97 === Abstract
Student ID:M9514007
Title of Thesis: Effect of Supplementation of Antibiotic-free Growth
Promoters on Growth Performances and Intestinal
Properties of Broilers
Total Page:76
Name of Institute: Department of Animal Science, National Pingtung University of Science and Technology
Graduate Date: June, 2009 Degree Conferred: Master
Name of Student: Tzu-Yih Huang Adviser: How-Hong Hsieh, Ph.D.
The Contents of Abstract in This Thesis:
The objectives of this experiment were to investigate the effects of supplementation of antibiotic-free growth promoters on growth performances, hemagglutination test, blood biochemistry value and intestinal properties of broilers. Two hundred and forty day-old broiler chickens were randomly assigned to four treatment groups: basal diet and basal diet supplemented with either 55 ppm tylosine (antibiotic), 50 ppm Bacillus subtilis (probiotics), or 0.1% PG-MIX (immune stimulator, peptidoglycan). Each treatment consisted of 3 replications. Feed and water were provided ad libitum. Birds were weighed individually, feed consumption, feed conversion ratio (F/G) and mortality were recorded at 21 and 42 days of age. In Exp.1 at 28d of age, 2 birds from each treatment were injected of sheep red blood cell (SRBC) for determining the antibody titer of hemagglutination test (HA test). In Exp.2 at 42d of age, 2 birds from each treatment were sampled for serum cholesterol, triglyceride, immunoglobulin and morphology of ileal and jejunal mucosa test. The immunoglobulin concentration in sera were determined by enzyme-linked immunosorbent assay (ELISA). The results from both experiments indicated that the body weights at 42 days of age were not significantly different among four treatment groups. However, the group treated with 50ppm Bacillus subtilis had better 42d body weight than the basal diet group. The antibody titer of HA test was maintained higher at 14 days after injecting SRBC for birds treated with PG-MIX. In Exp. 2, the triglyceride content of blood serum among all treatments were not significantly different. The total cholesterol concentrations of blood serum were significantly higher for birds treated with antibiotic and probiotics than the basal diet group (P < 0.05). Immunoglobulin densities were not significantly different among all treatments. The jejunal villus height and villus height/crypt depth ratio were significantly increased t with the birds treated with PG-MIX supplementation. In conclusion, the antibiotic-free growth promoter, has shown to improve the intestinal characteristics, prolong the serum antibody titer and increase the blood immunoglobulin density. Therefore enhanced the nutrient absorption, improved the intestinal health and increase the growth performances of broilers.
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