Effects of Formosan Sambar Deer (Cervus unicolor Swinhoi) Velvet Extracts on Gonadal Development and Reproductive Function in Male ICR Mice

• Main Authors: Chou, Meng-Hsien, 周孟憲
• Other Authors: Liu, Bing-Tsan
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/46524401274327353924

碩士 === 國立屏東科技大學 === 畜產系所 === 97 === Deer velvet has been a kind of traditional medicine which can strengthening the body. The purpose of this master thesis is to investigate the effects of the Formosan Sambar deer (Cervus unicolor Swinhoi) velvet extracts on gonadal development and the ability of testosterone secretion under the oxidative stress in vitro in the male ICR mice. The velvet were obtained from 4 3~5-year-old Formosan Sambar deer stags at 75 days after casting. One side of the velvet was divided into four sections (tip, upper, middle, and base) for phosphate buffer solution (PBS) and 20% ethanol extracts in fresh, the other side was preserved in -20℃ for 3 month in vacuum package for further extraction using the above-mentioned method. All the velvet extract samples were stored in -80℃ for the gonadal steroids (testosterone, progesterone, and estradiol) assay (Study 1), testing the effects on development of the reproductive organ and hormonal function (Study 2), and the semen quality (Study 3). It is showed that the PBS extracts contained estradiol higher than that in the 20% ethanol extracts, and the testosterone and progesterone contents in the 20% ethanol extracts, were higher significantly than that in the PBS extracts in the Formosan samber deer velvet, no matter what divided section or storage method (Study 1). Orally or injection administration of the Formosa sambar deer velvet PBS or 20% ethanol extracts to the male ICR mice in growth stage (weaned to puberty) benefited the reproductive organ development and serum testosterone concentration (Study 2), and the semen quality (Study 3).