Cloning and expression of cannabinoid receptor 1
碩士 === 國立屏東科技大學 === 食品科學系所 === 97 === The cannabinoid receptor 1 (CB1) is a membrane of the G protein-coupled receptor superfamily, which is located in the brain, and also in some peripheral tissues. CB1 affect various behavioral processes, including emotion, learning and memory, and mediating most...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2009
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| Online Access: | http://ndltd.ncl.edu.tw/handle/68037713203383898765 |
| Summary: | 碩士 === 國立屏東科技大學 === 食品科學系所 === 97 === The cannabinoid receptor 1 (CB1) is a membrane of the G protein-coupled receptor superfamily, which is located in the brain, and also in some peripheral tissues. CB1 affect various behavioral processes, including emotion, learning and memory, and mediating most of the neurobehavioral effects of physiological ligands. CB1 knock-out mice have been shown to be more susceptible to developing depressive-like behaviour. The pharmacological enhancement of physiological ligands activity at the CB1 cannabinoid receptor level appears to exert an antidepressant-like effect in some animal models of depression. The genes encoding human, mouse and rat CB1 have been cloned and expressed in mammalian cell system. Western blotting and Confocal microscope assay confirmed that CB1 was expressed on the cell surface and ligand-binding ability. The surface expression of CB1 was used to establish the competitive affinity assay. This study also investigated the relationship between the specific structures of herbs and their affinity to human, mouse and rat CB1 in order to obtain herbs with high affinity from different sources and structure for improving human health.
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