Detoxifying Enzymes Induction Effect of Methyl Gallate in Human HepG2/C3A cells

• Main Authors: Tsuei-Ru Liou, 劉翠茹
• Other Authors: Tzou-Chi Huang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/82204328791195210696

碩士 === 國立屏東科技大學 === 生物科技研究所 === 97 === Detoxifying enzymes system protects the body against the potential harmful exposure to xenobiotics from the environment as well as certain endobiotics. Plant phenols contribute significant biological properties such as anti-inflammatory, antioxidant. Methyl gallate is the major phenol constituents of a lot of nature plant. It was reported that ROS as intracellular signaling molecules could activate of transcription factor NF-E2-related factor 2 (Nrf2) and bind to ARE sequences, which is leading to transcriptional activation of phase II enzymes including GCL and HO-1. This study is designed to assess the effect of methyl gallate induces phase II detoxifying enzymes through activation Nrf2 in HepG2/C3A cell lines. The results showed that methyl gallate has no cell toxicity in HepG2 cell lines under 100μM by MTT assay. We found that methyl gallate enhanced intracellular ROS and activated signaling factor on PI3K and MAPK pathway. In addition, methyl gallate induced Nrf2 translocation into nucleus and activated expression of Nrf2-regulated protein including HO-1, GCL and GLO-1. Eventually, methyl gallate can increase GSH content at 24 hour. This study demonstrated that methyl gallate enhances ROS-induced phase II detoxification enzymes expression.