| Summary: | 碩士 === 國立屏東科技大學 === 水產養殖系所 === 97 === The parameters such as growth performance, liver antioxidant enzyme activity, liver detoxifying enzyme activity, and serum cholesterol content and triglyceride content were evauated when Japanese eel (Anguilla japonica) were fed diets containing Monostroma nitidum powder with 0%, 3.0%, 6.0%, and 9.0% added into feed as tested groups during 8 weeks. Results indicated that eel fed a diet of 6.0% group for the growth performance, had significant high HSI (Hepatosomatic index) but with no significant differences among the other parameters. The survival of eel that fed a diet of 6.0% and 9.0% groups was low at 69% and 75%, respectively. For eels fed diets of 3.0% and 9.0% groups showed a significant decrease in their serum cholesterol content. A significant reduction of the serum triglyceride content was observed for diets of 3.0%, 6.0%, and 9.0% groups.
The results marked that GSH (Glutathione) content and antioxidative enzyme activity of GSH Rd (Glutathione reductase) had no significantly difference among all tested groups. For CAT (Catalase) activity, those fed 3.0%, 6.0% and 9.0% groups exhibited a significant higher activity than that of 0 % group. GST (Glutathione S-transferase) activity for eels fed 3.0% group was significantly higher than other groups. GSH-Px (Glutathioine peroxidase) activity for 6.0% and 9.0% groups were significantly higher than that of 0% group. SOD (Sueroxide dismutase) activity for 0% and 9.0% groups were significantly higher than other groups. The lowest SOD value was found for eels fed 3.0% group.
After Cd2+ acute toxication (0.69 mg/L, 48 hrs), CAT activity for eels fed 6.0% and 9.0% groups had significantly higher value than other groups. The highest value of GST activity was observed for eels fed 9.0% group. SOD activity for eels fed 6.0% and 9.0% were significantly higher than other groups. For groups added of 6.0% and 9.0% Monostroma nitidum powder would significantly increase the antioxidant activity of Japanese eel. In addition, under acute toxication, it may effectively eliminate the superfluous free radicals and protect cell from injury in eel.
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