Investigating Antidiabetic Activity of Compounds from Anemarrhena asphodeloides

• Main Authors: Chien-Yi Lu, 呂建億
• Other Authors: Huey-Lien Kao
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/pp3u58

碩士 === 國立屏東教育大學 === 應用化學暨生命科學系 === 97 === Anemarrhena asphodeloides (AA) has been used as a traditional Chinese medicine for Diabetes mellitus (DM). Experimental antidiabetes method was inhibitor of α-glucosidase. The initial result exhibited 50% of α-glucosidase inhibitory activity from AA(dried rhizome) were extractd with MeOH. After AA removed tannin by polyamide adsorption exhibited more than 90% of α-glucosidase inhibitory activity. Active compounds of AA(1Kg) were extracted with the MeOH for five times. The crude extract was partitioned between MeOH/Hexanes, H2O/Ethyl Acetate, and H2O/Butanol. Four compounds were isolated from each partitioned fraction of AA by chromatographic methods. The structure of three compounds were determined by spectral analysis and chemical transformation. They are mangiferin, 2,6,4’-trihydroxy-4-methoxybenzophenone and nyasol. The other compound under defining is unknown. In addition, the pharmacological activities of AA on DPPH radical scavening and anti-tyrosinase was also investigated.