Effects by Pregna-1,20-dien-3-one, a Novel Bioactive Steroids, from the Soft Coral Umbellulifera striata in human leukemia HL-60 cells.

• Main Authors: Yi-Zhang Zhang, 張懿政
• Other Authors: Ying-Tang Huang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/96284164428934414701

碩士 === 國立高雄海洋科技大學 === 水產食品科學研究所 === 97 === Pregna-1,20-dien-3-one (Pregna), one of pregnane steroids, was obtained from column chromatography of chloroform extract of Umbellulifera striata on Silica gel. Our results showed the Pregna exhibited more growth inhibitory effect on HL-60 than other tested human leukemia cells, such as U937, THP-1, Jurkat. Pregna inhibited cell proliferation and induced apoptosis in a concentration- and time-dependent in HL-60 cells. Treatment of HL-60 cells with pregna, it resulted in dissipation of mitochondrial membrane potential, release of mitochondrial cytochrome C into cytosolic, activation of caspases 9 and -3, disruption of DNA integrity, and induction of apoptosis Moreover, treatment with Pregna caused a rapid loss of intracellular glutathione content and stimulation of reactive oxygen species (ROS) generation. N-acetylcysteine (NAC), an antioxidant, and the pan-caspase inhibitor, z-Val-Ala-Asp-fluoromethyl ketone (z-VAD-FMK), significantly inhibited Pregna-induced cell death. Taken together, it suggests that induction of apoptosis by Pregna may be modulated by generation of ROS and activation of caspase activity. According to our results, Pregna may be a promising anti-cancer drug and need to further study the molecular mechanism of anti-cancer effect.