| Summary: | 碩士 === 國立東華大學 === 應用物理研究所 === 97 === Nanometer-sized diamond has attracted increasing attentions recently. It is considered as a promising nanomaterial for bio-applications for its biocompatibility, easy functionalization/conjugation with biomolecules, superb optical and physical/chemical properties. Recently, it has been used as markers for cell imaging using its fluorescence or Raman signals for detection. While the bio applications look promising, many of the understanding on bio or spectroscopic properties upon interfacing with bio systems are still in their early stage. However, the activities of attached protein can be affected by the interaction with nanodiamond surface.
As a test model, protein lysozyme conjugation with ND provides an ideal case for study the protein activities and the spectroscopic properties of the ND-lysozyme. We concentrate on the physical adsorption (electrostatic interaction) method for conjugation of lysozyme to ND. In this work, we study the Raman, UV-Visible spectrometer, IR and photoluminescence properties of the prepared complex of nanodiamond (5-500 nm in diameter) with adsorbed lysozyme; and the
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