| Summary: | 碩士 === 國立東華大學 === 海洋生物科技研究所 === 97 === Regulatory mechanism of marine symbiosis in Cnidarian dinoflagellate association remains unclear notwithstanding decades of research. Symbiotic gastrodermal cell (i.e., SGCs) membrane may play important roles in the process of
initiation and maintainence of this endosymbiosis. In order to explore the interactions between symbionts and their host, investigation of the features of plasma membrane between gastrodermal cells and symbiont is prerequisite. Here, we first examine cell surface proteins of SGCs by a chemical biotinylation process. By biotin-streptavidin has high affinity constant (KD = 10-15 M), the intactness of SGCs and successful protein biotinylation are shown by streptavidin fluorescence staining and observed by confocol microsopy. We identified these biotinylated proteins from SGCs by 2D SDS-PAGE. Many peridinin chlorophyll binding proteins were identified. Nevertheless, two-dimensional distribution pattern of biotinylated proteins of symbiotic gastrodermal cells show significant difference from that of cultural Symbiodinium. By comparison of the fluorescence image, biotinylated proteins identification of SGCs, and biotinylated protein pattern between SGCs and cultural Symbiodinium, it revealed plasma membrane of SGC may have unique channel or mechanism to permit the biotin molecule across the multiple membranes into endosymbiotic Symbiodinium which was then labeled. However, these phenomenons were not found in cultural Symbiodinium. Therefore, in this study was suggested that plasma membrane of gastroderm involve unique interesting characteristics and functions such as nutrients transportation, and it plays important rules to modulate coral-dinoflagellate endosymbiosis.
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