| Summary: | 碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 97 === In recent years, micro total analysis system (μTAS) has become more popular in several biotech fields. However, manipulating and selecting single cell in a defined location is an important issue in sample analysis of pre-treatment procedures.
This paper presents a laser micro bubble system method for single cell selecting, which sample is Human Histiocytic Lymphoma cells. To achieve the purpose of selecting single cell, we examined for several procedures such as: (i) the situation of U type and cycle type channel trapping single cell, (ii) the injection of ink fluid in laser heating area of our device and (iii) fiber laser heating, to produce micro bubbles to push the cell which is trapped by micro structure channel.
According to experiments data, U type and cycle type channel can easily trap cells. However, U type channel’s trap range is parallel to the direction of cell flow. It is easily let many cells pile up on trapping location. And cycle channel, which trap range is vertical to the flow direction, has a better capability for trapping single cell. On the other hand, using laser heating which power is above 0.9W, can successfully produce micro bubbles to push cell out of structures less than 1 second.
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