| Summary: | 碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 97 === This research is using electrophoresis force by driving voltage of electrodes to study the cell adhesive ability of human dermal fibroblasts (HS68) on polydimethylsiloxane (PDMS), polycaprolactone (PCL) and polylactide-co-glycolide (PLGA) materials which have different biodegradation rate.
In this research, we studied cell proliferation rate of fibroblasts on different material surface, measured cell adhesive force by electrophoresis and analyzed cell spreading area with image process software with different generations of fibroblasts to investigate the relationship between cell spreading area and cell adhesive force. In addition, oxygen plasma is applied in this experiment for modifying the wettablity on the surface of materials and then evaluating the tendency of cell adhesion with the change of contact angle.
From the cell proliferation study, cell adhesive force analysis and the measurement of cell spreading area, fibroblasts cultured on PLGA surface had the best cell attachment in comparison with PCL and PDMS. Overall, PLGA materials have better biocompatibility and wettability.
The application of oxygen plasma can modify the surface of biomaterials by varying the function groups of the polymers. The results of this experiment represent that the material surface with a contact angle of 50-55o had the best cell attachment and proliferation rate.
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