| Summary: | 碩士 === 國立嘉義大學 === 農業生物技術研究所 === 97 === Abstract
Genetic mapping is a powerful strategy to determinate the location of genes on chromosomes via the usage of molecular marker systems and segregation populations. Initially the mapping informations show that the level of linkage relationship between markers and trait of interest can be established. According to the results, the markers linked to specific traits can be used for further map-based cloning to obtain the target gene. In addition, the linked information also can be immediately used for marker-assisted selection in traditional breeding to accelerate its efficiency due to rapid, convenient, and large-scale selection at any growth stage of plants. So far, there are some commonly-used polymorphism-detecting systems for mapping usage including of restriction fragment length polymorphisms (RFLP), amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD) and simple sequence length polymorphism (SSLP).
The goal of this experiment is to identify molecular markers linked with heat-tolerant traits. Based on previous studies, 5 SSR markers were used for marker-assisted selection and distinguished tomato lines into two catalogues of heat-tolerant and –sensitive lines. Otherwise, the AFLP method with 100 primer combinations was initially used to detect polymorphism difference among two parental lines and F1 plants, then selected 41 primer combinations of them were used to detect molecular polymorphism among 65 individuals in a F2 population.
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