Female-Specific DNA Marker in Cacatua Galerita for Sexing

• Main Authors: Yun-Hsia Cheng, 鄭雲霞
• Other Authors: Chean-Ping Wu
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/63752065074303312492

碩士 === 國立嘉義大學 === 動物科學系研究所 === 97 === Sulphur-crested Cockatoo (Cacatua galerita) distributes in New Guinea and Australia. Its length is between 45 and 55 centimeter. The crest’s color is yellow and the feather of body is white. It’s difficult to identify the sex of Sulphur-crested Cockatoo (Cacatua galerita) by the external morphology. This study was conducted to investigate the RAPD fingerprints of Sulphur-crested Cockatoo by RAPD-PCR, and establish a way to identify the sex by PCR. There are one hundred random primers were used for RAPD fingerprinting, and one of these primers produced a 579 bp sex-specific band in the female DNA fingerprints only. Two primers (CGSEX-F & -R) were designed according to the cloned sequence for amplifying the female-specific band by PCR for Sulphur-crested Cockatoo sex identification. The 18S ribosomal gene was used as an internal control. The results showed that sex-specific bands (579 bp) in the gel could be observed in all females but not in males. The genomic DNA of Myiopsitta monachus, Trichoglossus haematodus, Poicephalus robustus, Poicephalus gulielmi, Aratinga wagleri, and Aratinga mitrata amplified with CGSEX-F & -R, and the sex-specific bands obtained are 695, 567, 572, 571, 981 and 981 bp respectively. All of the sex-specific bands do not show in males. The sex-specific primers were also used for sexing the non-parrot birds (Cotumix japonica, Gallinula chloropus, pigeon, and chicken), the results showed they don’t have any sex-specific bands amplified. In conclusion, the sex of Sulphur-crested Cockatoo, Myiopsitta monachus, Trichoglossus haematodus, Poicephalus robustus, Poicephalus gulielmi, Aratinga wagleri, and Aratinga mitrata could be accurately and rapidly identified by CGSEX-F & -R by PCR, but can’t apply to non-parrot birds. The CGSEX-F & -R primers are specific to parrots for sexing.