| Summary: | 碩士 === 國立嘉義大學 === 生化科技研究所 === 97 === Neurogenesis is the process of newly generated neurons. This occurs mainly in the subventricular zone (SVZ) and the subgranular zone (SGZ) of the hippocampus. Neurogenesis in adult hippocampus has been suggested to correlate with certain aspects of brain cognitive function, including learning and memory. Traumatic brain injury (TBI) is one of the most prevalent causes of morbidity and mortality all over the world. TBI results in neurological dysfunction and death through primary or secondary mechanisms. Thermal stimulation (TS) has been reported to significantly enhance the recovery of several aspects of sensory and motor functions in stroke patients. In this study, we aimed to evaluate the effect of TS on the learning and memory and its neurogenic mechanism after TBI. Double-immunochemistry was used to detect the newly produced neuron and Morris water maze (MWM) was performed to test the spatial memory after TBI. The present study demonstrated that TS treatment after TBI, in 2 weeks, could significantly enhance the level of 5’-bromodeoxyuridine (BrdU) and doublecortin (DCX) positive cell in rat hippocampus. TS also improved the learning and memory performance and attenuated the tissue damage after TBI. Our previous study has evidenced that VEGF is essential for neurogenesis after TBI. The present results have further proved that the administration of VEGF antisense could significantly attenuate TS induced neurogenesis and spatial memory recovery, suggesting that the effect of TS is also mediated by VEGF.
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