| Summary: | 碩士 === 國立彰化師範大學 === 生物學系 === 97 === Nitric oxide is a very important biological substance in mammals. It participates in the nervous system of the signal transmission, immune response, and promotion of vasodilation. In recent years, studies have shown that the amount of nitric oxide could affect the growth of cancer cells. High doses of NO (> 1 micorM) could lead to the death of cancer cells. However, in normal physiological conditions, the concentration of nitric oxide is very low. Therefore, several NO donors have been developed and used in cancer research. Curcumin is a natural polyphenol which has been proved in clinical trial for inhibiting tumor growth. However, the low solubility and poor absorption by oral administration have reduced the clinical value of curcumin. Recent researches have focused on co-treatment of curcumin and other compounds to increase the cytotoxicity curcumin. The aim of this study is to investigate the cytotoxicity of curcumin combined with two types NO donors: DNICs (dinitrosyl iron complexes) and RREs (Roussin’s red esters) against mouse melanoma cells (B16-F10). Eight NO donors (NC01、02、03、04、06、08、10、11) were tested in this study. The results showed that NC03, NC04 combined with curcumin could reduce the cytotoxicity of curcumin. But, the pretreatment of curcumin for 4 hrs followed by the treatment of NC03 for 24 hrs could increase the cytotoxicity of curcumin. However, pretreatment of NC03 for 4 hrs followed by the treatment of curcumin for 24 hrs did not enhance the cytotoxicity. Furthermore, the co-treatment of the low concentration NC10 and curcumin displayed significant increasing cytotoxicity. The results of UV spectra analysis suggested that NC03, NC04 and NC10 may interact with curcumin, therefore, altering cytotoxicity of curcumin. Together, these results provide useful information for the development of new curcumin- NO donors complexes as anti-cancer agents in the future.
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