| Summary: | 碩士 === 國立中央大學 === 機械工程研究所 === 97 === The study design a pressure bioreactor, in order to steady and correct pressure value, we frame automatic control hydrostatic system that can provide constant and time-periodic pressure for in vitro cell cultures. The systems’ performance was stable at a constant hydrostatic pressure up to 5 MPa and cyclic hydrosatatic pressure up to 1 Hz. Cells were putting in the whirl-Pak Sample Bags, which could largely reduce the amount of culture media and prevent contamination.
We tested the rat bone marrow stem cells (RM1) by applying 0.4, 0.55, 0.7 and 1 MPa of static pressure at the bioreactor. We study how the pressure slope in time may influence the cell growth by, we changed the pressure slope for the 1 MPa case. The results show it is likely to be the pressure slope rather than the final static pressure value that inhibited the RM1 growth. The cells growth apparently slowed down when the pressure slopes were large than 700 KPa/s. The bioreactor was proved to be available in cells culture and it will be applied to test cells for gene expressions in the future.
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