Development of the Biospecimen Storage System with Liquid Nitrogen and Real-time monitoring System

• Main Authors: Chun-Lung Huang, 黃俊隆
• Other Authors: Shang-Chih Lin
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/92277945398781608033

碩士 === 國立中央大學 === 機械工程研究所 === 97 === ABSTRACT The progress of biotechnology not merely advents the human welfare, but reveals great economic potential. With the development of technology and the attraction of business profits, the biotechnology is destined to become a nouveau high-tech industry where the whole world will pour into enormous efforts. This study aims to develop a container with liquid nitrogen at -196°C as a more suitable storage for biological samples, by extracting advantage and eliminating limitation from currently-adopted containers. At present, there are the traditional manual lid-opening container by Taylor-Wharton, the mechanical container by Cryo-Cell (Cryo-Cell International Inc., USA), the one by BioArchive (Thermo genesis Corp., USA) and others in Taiwan. The study procedure include hardware and software design, hardware: In addition to the 3D computer model’s changes, entities processed and equipment’s actual test of using of the first-generation storage system, while also continue to develop the design of the second-generation storage system. After the completion of the mechanical structure design, we calculate the structural strength with the finite-element analysis in order to understand whether the stress concentration exceeds the material strength and cause fracture of structure. Software: TO develop an operation and control system with human-machine interface and a sensor’s real-time surveillance system for storage’s security. Research results: the actual test of using and analysis results show that the design of such storage system is viable. With the simplest design, the storage system can just access one single biospecimen for each time and avoid to effect the biological activities of other samples. Finally, to cooperate the operation and control with human-machine interface system and real-time surveillance system.