Study of the Effects of Shear Stresses on the Growth and Morphology of Rat Bone Marrow Stem Cells with the Cone-Plate Bioreactor

• Main Authors: Da-Shiang Ma, 馬大翔
• Other Authors: Chih-Ang Chung
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/43455985882715803637

碩士 === 國立中央大學 === 機械工程研究所 === 97 === We designed a cone-plate bioreactor. It produces fluid shear stress by rotating the cone that drives the culture medium to flow in a circular way. Rat bone marrow stem cells were cultured on coverslips placed on the plate. The cells experienced the shear stress of 1.4、5.5、11、16.4 dyn/cm2 respectively for 4 hours in 1 day and 14 hours in the bioreactor. Results showed the shear stresses changed the morphology and inhibited the cell growth when the shear magnitudes were over 1.4 dyn/cm2. We also found that, by applying shear stress of 1.4 dyn/cm2 for 4 hours within 3 days and 14 hours, the cells became elongated in shape and aligned to each other. The study demonstrates that the cone-plate bioreactor can provide well-defined stimulus to the cells cultured in vitro. The growth inhibition and shape changes of the cells by shears shall merit further research efforts to classify the underlying mechanisms and biochemical pathways.