The Research of Fluorescence Reaction Bio-sensor and its Application in Human Serum Albumin

• Main Authors: Chun-Yu Liao, 廖君宇
• Other Authors: Yue-ming Hsin
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/87299411549122077154

碩士 === 國立中央大學 === 電機工程研究所 === 97 === In this research, we present bio-sensors fabricated by the semiconductor fabrication and biomark techniques for sensing of Human Serum Albumin (HSA). The GaAs was used as the sensing material to fabricate heterojunction phototransistor array as the sensing device, and the fluorescence phenomenon of the biomark compound was designed as the signal. By combining the sensing device and biomark, a biosensor for HSA detection is demonstrated. Numerous of experiments were tested for the sensitivity and reproducibility of the fabricated sensors. For the reproducibility test, the conditions at the same concentration of HSA were taken for the detection, and the standard deviation of the results was only 3.39 × 10-9 A. And for the sensitivity test, the experiments showed that the sensor demonstrated a linear result in the increase of photo-induced current at the HSA solution concentration of between 0.01 mg/mL and 0.07 mg/mL. Moreover, a micro-flow system of integrating the sensor-array and feed-through setup was established, the experiments showed similar results to the single sensor. The results present a trend line of Y = 1.6 × 10-6 + 1.38 × 10-8X, where Y is the magnitude of photo-induced current, and X is the concentration of HSA solution. It shows that the magnitude of photo-induced current is increased by 13.8 nA when the concentration of HSA solution increases a microgram between 0.01 mg/mL to 0.07 mg/mL.