Functional analysis of CCR4 genesin rice-generation of overexpression and RNAi knock down transgenic rice plant

• Main Authors: Haw-En Ho, 何浩恩
• Other Authors: Chung-An Lu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/31979640219778323173

碩士 === 國立中央大學 === 生命科學研究所 === 97 === In general, gene expression is regulated at either a transcriptional level or a post-transcriptional level, or even both. In eukaryotes, the major mechanism of mRNA degradation involves a poly A tail deadenylation. The deadenylation is the rate-limiting step in many mRNA degradation events in a wide-range of organisms. In yeast, the CCR4 (carbohydrate catabolism repression 4) protein, a part of the CCR4-NOT complex, have been proposed that play a prominent role as deadenylase in mRNA degradation. However, the function of CCR4 in plants is not clear yet. In this study we use the monocot model plant, rice, to further investigate the general role of plant CCR4 in mRNA degradation. The OsCCR4-1 and OsCCR4-2 overexpression and RNAi knock down transgenic rice were generated, and they will be analyzed for the functions of OsCCR4-1 and OsCCR4-2 in rice subsequently. The T1 RNAi plant and T2 overexpression rice plant have been identified. Continuously we will identified the T2 transgenic plant and T3 homologous transgenic rice plant.The function of OsCCR4-1 and OsCCR4-2.will be analyzed in the future.