| Summary: | 碩士 === 國立交通大學 === 奈米科技研究所 === 97 === In order to find the ways for controlling the cell-material interface, we made two different topographies of one dimensional (1D) periodic lines/space pattern and two dimensional (2D) arrayed pillars pattern for cell behavior analysis. Here we used lithographic techniques can control not only the topographic pattern but also the scale of such topography within microscale ridge widths (~1�慆) and submicron deep grooves (~350nm). We investigated the microscal topography regulated cell functions using human epithelial carcinoma (HeLa) cell culture on poly(dimethylsiloxane) (PDMS), the silicon substrate with microstructures on it were used as templates for micromolding a silicon elastomer, PDMS, into tissue scaffolds for cell patterning purpose. We observed that on 1D periodic lines surface cells tend to align with the direction of microscale ridges and grooves and have better attachment through an integrin ��5 subunit expression compared with 2D periodic pillars pattern or flat PDMS surfaces. By contrast, cells cultured on the 2D periodic pillars and smooth PDMS substrates were mostly round and worse adherent with higher filopodia protruded, tumor suppressor protein 53 (p53) increased and matrix metalloproteinase-9 (MMP-9) expression. Considering the important role of integrin-mediated intracellular signaling in anchorage-dependent cell function, we found the external topography regulate cell function as cell growth, DNA synthesis, motility, and apoptosis. Modulation of cellular morphology related integrin-mediated signaling by altering substrate topography will have useful applications in biomaterials science and tissue engineering.
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