| Summary: | 博士 === 國立交通大學 === 應用化學系所 === 97 === Abstract
The study of this dissertation is based on electrochemistry, including the modification of nano-materials on electrode for electrochemical detection, and the fabrication of microchip capillary electrophoresis (CE) in conjunction with electrochemical detection. In the part of modification of nano-materials on electrode, the platinum nanoparticles were used for the surface modification on working electrode by electrodeposition. In the optimal conditions of electrodeposition, the platinum nanoparticles modified electrode performed good detecting results for ethanol sensing. The performances of this modified electrode included good sensitivity, response time, selectivity, and reproducibility. The response time and sensitivity for different type of alcohol samples, including alcoholic beverages and human serum, was measured to evaluate the selectivity. The storage stability of the sensor was also evaluated. These characteristics allowed its application for direct detection of ethanol in biosensing and controlling.
The applications of microchip CE are getting extensively. Microchip CE is characterized by a number of analytical advantages, including its rapidity, a small sample requirement and the potential for integration. However, the fabrication of microchip CE, including the chip material and types of detection, are needed to be improved. A dry-film photoresist-based microchip using a combination of photolithographic and hot-roll lamination techniques was employed in this study to improve the fabrication of microchip. The microchannel pattern was prefabricated in a dry-film photoresist tape using photolithographic methods. The integrated microchip device was then fixed in a laboratory-built Plexiglas holder prior to its application to microchip electrophoresis. The performance of this device as an electrochemical sensor was evaluated for the separation of neurotransmitters. The fabrication procedure described in this study is time- and cost-effective; it has great potential for use in the design and prototyping of disposable microscale analytical systems for a range of chemical and biochemical separations. A microchip integrated with electrochemical sensor which uses indium tin oxide (ITO) film electrodes as a working electrode and a decoupler was developed. The ITO film on a glass substrate was patterned by photolithography and wet etching as the decoupler and working electrode. This fabrication provided a way to solve the problem that the off-channel electrochemical detector integrated with microchip. The performance of this electrochemical sensor was evaluated by the separation of catecholamine. Excellent efficiency and resolution were obtained. This approach has a great potential for use in the fabrication of off-channel electrochemical microscale analytical systems for chemical and biochemical separations.
|
|---|
