Systematic Analysis of miRNA Regulations in Tumor Cells

• Main Authors: Hsu, Paul Wei-Che, 徐唯哲
• Other Authors: Huang, Hsien-Da
• Format: Others
• Language: en_US
• Online Access: http://ndltd.ncl.edu.tw/handle/51573551973191605885

博士 === 國立交通大學 === 生物資訊研究所 === 97 === MicroRNAs (miRNAs) are small non-coding RNA molecules of ~22 nt sequences that have an important role in the translational inhibition and degradation of mRNA to downregulate gene expression. Recent work supports miRNAs downregulate gene expression during various crucial cell processes such as apoptosis, differentiation and development. Recent studies have suggested that oncogenesis may be link with aberrant expression of miRNAs, but in most case it is still not clear what mechanism of miRNA leads to cancer formation. In this study, we analyzed more than 200 miRNAs which are aberrantly expressed in tumor cells, and the tissue specificity is tested by the miRNA expression among normal tissues. We identified 20 oncomirs which are up or down-expressed to regulate downstream genes and involved in oncogenesis of eight cancer types. We also predicted the transcription factor binding sites (TFBS) in miRNA promoter and identified miRNA targets which are tumour suppressors or oncogenes. Those analyses are integrated for miRNA regulatory network construction in eight cancers, and we hope our achievements can support cancer research and clinical trial.