Antibodies in the sera of patients with Helicobacter pylori infection promote the activities of pathogenic heat shock protein 60 to induce the expressions of proinflammatory cytokines

• Main Authors: Yang, Chu-Ting, 楊筑婷
• Other Authors: Liao, Kuang-Wen
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/57329672533738279700

碩士 === 國立交通大學 === 生物科技系所 === 97 === H. pylori-derived heat shock proteins (HSP60s) are closely associated with gastric inflammation to induce the expressions of proinflammatory cytokines. In clinical examination, anti-HpHSP60 antibodies could be detected in H.pylori-postive patients’ sera. Surprisingly, the sera from 16 patients with gastric cancer, gastritis, duodenal ulcer or peptic ulcer could not neutralize the activities of recombinant HpHSP60 to induce releases of TNF-alpha and IL-8 but they raised the expressions of proinflammatory cytokines. The results showed the no matter what species’ anti-HpHSP60 polysera all could cause an enhancive effect on HpHSP60 to induce TNF-alpha or IL-8 expression. Furthermore, the specific antibodies in sera but not other factors or non-specific antibodies contributed to the enhancive effect. Finally, the results suggested Fc receptor plays a role in the enhancive effect of anti-HpHSP60. Together these results, we explored that the patients’ anti-HpHSP60 antibodies in sera may result in more serious inflammation in the presence of HpHSP60. This finding may provide further application in clinical treatments for the patients with H. pylori infection.