Generation of transgenic Brainbow Drosophila for multicolor labeling of cells

• Main Authors: Yu-Chen Liu, 劉育臣
• Other Authors: 傅在峰
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/12957217449069648833

碩士 === 國立暨南國際大學 === 應用化學系 === 97 === Thousands of genes operate in the brain, each at a different space and time, to control complex behavior in an animal. Understanding how the brain to operate has long been a formidable challenge in neuroscience research. A key step towards understanding the development and function of the central nervous system is by characterizing the connections among neurons, exceedingly complex and yet precise in the central nervous system. These wiring patterns are characterized by specific synaptic connections. Interactions among synaptic inputs enable neurons to compute the overall consequence of various stimuli and provide the cellular basis of cognitive processes and behavior. Here, we proposed using and modifying the “Brainbow” techniques to understand the wiring diagram in Drosophila brain. The “UAS-fly Brainbow” transgenes (named the UAS-ACMY or UAS-MCY), frt/Flp is used to create a stochastic choice of expression some different fluorescent proteins (XFPs; ACMY by sequencial A=AcGFP, C=CFP, M=mKO, Y=YFP and the MCY by sequencial M=mKO, C=CFP, Y=YFP) and the multiple copies of transgene in transgenic fly under control by Gal4 and Flp in transgenic fly yielded combinatorial XFPs expression. To validate generated “UAS-fly Brainbow”, for the first time, we developed a new tool to illustrate the neural circuits in Drosophila brain.