Prolong Antibiotics Release by Encapsulating PLGA for Hip Prosthesis

• Main Authors: Kai-hsiang Chen, 陳凱翔
• Other Authors: Ming-long Yeh
• Format: Others
• Language: en_US
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/87162802323635487176

碩士 === 國立成功大學 === 醫學工程研究所碩博士班 === 97 === Deep infection after artificial joint arthroplasty is considered as one of　the most devastating complications. Patients with deep infection may suffer from multiple surgeries and long period of hospitalization. In this study, encapsulating antibiotic loaded titanium alloy hip prosthesis materials by biodegradable poly(lactic-co-glycolic acid) copolymer (PLGA) were fabricated to extend period for steady antibiotic release and prevent burst antibiotic release. Two antibiotics (Vancomycin and Cefuroxime) and different concentrations and layers of PLGA encapsulations were investigated to find the optimal antibiotic release. The antibiotic release was quantified with elution test by immersed the samples in Phosphate buffer solution (PBS) then shaken with rotator. At 1, 6, 12, 24, and every 24 hours, extracts from eluting PBS were collected for antibiotic concentration analysis by spectrophotometer and replaced with fresh PBS. The antibiotic release was correlated with the degradation of PLGA. Weight change, pH value and metallographic microscopy were used to investigate PLGA degradation. The results shows the effective antibiotic release for single layer 15% PLGA on Vancomycin group, single layer 15% PLGA on Cefuroxime group, double layers 15% PLGA on Cefuroxime group, and one layer 30% over one layer 15% PLGA on were 5, 7, 10 and 17 days respectively. Cefuroxime has a longer drug releasing period than vancomycin in this PLGA encapsulating approach. In conclusion, this biodegradable PLGA encapsulating antibiotic strategy can extend antibiotic release up to 2.5 weeks in fast in vitro elution test. It could be further extended the release to longer time in vivo to reach the clinical effectiveness.