| Summary: | 碩士 === 國立成功大學 === 環境醫學研究所 === 97 === This research is aimed to investigate the relationships between urinary monoester metabolites and the PAE levels in house dust for precocious puberty girls and normal girls. The demographic characteristics and exposure scenario including dietary ingestion, personal care products and cosmetic usage for subjects were obtained from an interviewed questionnaire. The precocious puberty girls were recruited from the policlinic of Pediatric Endocrinology in National Cheng Kung University Hospital and normal girls were recruited as control group. The urinary monoester metabolites of subjects and the phthalate ester levels in house dust were measured by HPLC-MS/MS and GC/MS, respectively. The analytic results from all house dust samples in four different areas shows that DEHP (834.7~1643.6 mg/kg) is the dominant compound in all samples, followed by DBP (21.5~34.4 mg/kg) and BBP(3.03~4.16 mg/kg). DEHP contributes over 96% of total PAE concentration in house dust samples. The levels of DBP in the dust from bed of precocious puberty girls were significantly higher than those from normal girls (P=0.02). In total, the levels of DEHP in house dust in Taiwan were 2.3-4.8 folds higher than those in German, US and Norway. The highest PAE metabolites in urine samples from all subjects were MBP (78.1 µg/g creatinine), followed by MEP (18.7 µg/g creatinine), MEHP (12.9 µg/g creatinine), MMP (9.09 µg/g creatinine) and MBzP (5.25 µg/g creatinine). The levels of MEP in precocious puberty girls were significantly higher than those in normal girls(25.5 vs.10.9 µg/g creatinine，P=0.013), while the MBP was on the contrary. In general, the internal dose of MBP in this study groups were 2 times higher than American children and the internal dose of MEHP were 2.5 times higher than American children and German childen, respectively. In the dietary comsumption survey, the consumption quantities of beef, mutton, milk products, and hot dog in precocious puberty girls were higher than normal girls. The plastic packaged convenient foods were consumed more frequently in precocious puberty girls than normal girls. All subjects were divided into high and low PAE level groups by median level of house dust and adjusted-urinary samples. The data shows that the high PAEs in house dust group used more plastics materials as flooring material than low level group. The consumption quantities of off-premises eating, off-premises drinking and the usage of personal care products in high urinary PAE group were higher than low PAE group. Futhermore, there was no relationships between urinary monoester metabolite concentrations and dust PAE levels in precocious puberty girls. The daily intake dose (DIest) of five PAEs in all subjects were estimated from the level of urinary PAE metabolites based on the pharmacokinetics model. The calculated median DIest (µg/kg/day) of five PAEs in all girls were as followed: DEHP was 5.21(0.38-74.36) µg/kg/day, DBP was 2.69(0.77-15.54) µg/kg/day, DEP was 0.64(0.04-13.05) µg/kg/day, DMP was 0.31(0.03-4.42) µg/kg/day and BBP was 0.17(0.03-2.75) µg/kg/day, respectively.
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