Method Development of Molecular Imprinting Polymers for the Analysis of PAHs in the Mainstream Smoke from Cigarettes.

• Main Authors: Jiun-an Chen, 陳俊安
• Other Authors: Ta-cheng Lin
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/91914476367937432887

碩士 === 國立成功大學 === 環境工程學系碩博士班 === 97 === Polycyclic aromatic hydrocarbons (PAHs) and their derivatives are common trace pollutants produced during incomplete combustion of organic substances such as tobacco. After sampling cigarette smoke, PAH collators must undergo pretreatment processes such as extraction, cleanup and concentration before instrument analysis. This study combines molecular imprinted polymers (MIPs) and solid-phase extraction (SPE) to create a novel sample pretreatment technique. Experimental results demonstrate that MIPs have good selectivity for Benz[a]Pyrene (BaP) from a mixture of 16 PAH solvents. The MIPs were applied to a sample taken from mainstream smoke from a cigarette. Based on functional monomers and cross-linkers, this study investigated three groups of MIPs. After the template was removed, various tests, including capacity, selectivity, recovery, scanning electron microscope (SEM) observations and real environmental sample tests, were conducted. Experimental results show that MIP-1 is the best MIP with a capacity of 20.78 ± 1.7 (μg/g), BaP selectivity and recovery exceeding 93%. In environmental sample tests, i.e., main stream smoke from S and M brand cigarettes, 80% of BAP was absorbed by MIP compared with that absorbed by filters. All experimental results suggest that MIPs effectively adsorbed BaP among 16 different PAHs, reduced background interference and increased signal resolution compared with traditional extraction techniques. Additionally, using MIPs for sample pretreatment is less laborious than traditional sample pretreatments. It saves time and reduces organic solvent reduces consumption. Using molecular imprinted polymers solid extraction (MISPE) may separate target analytes from a complex sample more effectively than traditional Soxhlet extraction. Future toxicity tests should contribute to the understanding of toxic compounds emitted from specific pollution sources.