Effect of Permanganation on the Cell Integrity and Metabolite Release of Three Nauseous Cyanobacteria

• Main Authors: Yi-ting Chiu, 邱宜亭
• Other Authors: Tsair-Fuh Lin
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/91249854208041451438

碩士 === 國立成功大學 === 環境工程學系碩博士班 === 97 === The effect of permanganation on the cell integrity and metabolite release of three nauseous cyanobacteria, including Microcystis aeruginosa, Anabaena circinalis, and Cylindrospermopsis raciborskii, is investigated. In the experiment, the cyanobacteria were grown either in algae growth medium or in the filtrated water from Tai-Lake, Kinmen, with addition of algae growth medium. A fluorescence technique, combining fluorescein diacetate (FDA) with flow cytometer, was successfully developed for the determination of cell integrity for M. aeruginosa and C. raciborskii, while was not successful for A. circinalis. A solid-phade microextraction (SPME) concentration followed by a gas chromatograph (GC) and mass spectrometric detector (MSD) was employed to measure the metabolites from the cyanobacteria. A series of permangnation of cyanobacteria-laden water was conducted at different cell concentrations and different permangnate dosages. During the experiments, permanganate concentration, cell integrity, and metabolite concentration were monitored at different time. The experimental results revealed that the Cylindrospermopsis cells are more fragile to permanganate than Microcystis cells. At an initial permanganate concentration of 2 mg/L, cells number of 300,000 cells/mL, and reaction time of 60 minutes, 57% Microcystis cells, and 74% Cylindrospermopsis cells were ruptured in algae growth medium, and only 15% Microcystis cells were ruptured in the filtrated water from Tai-Lake. For Microcystis metabolite, both β-carotene oxygenases present in the cells and permanganate may be able to cleave the carbon-carbon double bonds of β-carotene, resulting in the formation of β-cyclocitral, Since β-cyclocitral may be degraded by permanganate, for longer reacton time (120 minutes in this case), the rate of degradation may become higher than that of formation, casuing a reduction of β-cyclocitral concentration in the samples. For geosmin, the major nauseous metabolite of Anabaena, may be formed by the geosmin sythetases in the cells with magnesium as catalyst. When Anabaena cells are ruptured, magnesium would have more chances to contact with the geosmin synthetases, resulting in the formation of geosmin. Since geosmin is resistant to permanganate, the total geomsin concentration in the samples did not change much in the permangnation experiments of Anabaena.