| Summary: | 碩士 === 國立成功大學 === 環境工程學系碩博士班 === 97 === Water is one of the most important necessities in human life. However, the order problem in the tap water has long become an annoying problem to solve, although order is a direct way to detect the quality of water. Various ways of water purification and deodorizations designed for reservoirs are proved to be effective but way too expensive in terms of installation and maintenance. The purpose of the study is to explore how Bacillus, separated voluntarily and identified through the molecular biological techniques in the laboratory, can help to degrade the order compounds in water when added with different substrate concentrations for co-metabolisms. The study is designed to find out the best proportion of the co-metabolism substrate addition.
The method of the study involved the bacteria culture (B-1 and B-2) and the five groups of different proportions of ethanol addition, from 0.2% to 1.0%. The research findings showed that two bacteria share similar growth cycle—growing massively 3 hours after being raised, with the growth generation for 10 hours. This results of the study indicated that adding the substrate for co-metabolism really enhanced the efficiency of order-compounds degradation by the test-bacterium. It is found that the degradation to 2-MIB is more efficient than to Geosmin. The best period of degradation is found to be 9 days, regardless the proportion of co-metabolism substrate. Moreover, when the ethanol proportion is 0.6%, the degradation reached the best effect for both bacteria (B-1 and B-2) to 2-MIB, with the efficiencies of B-1 and B-2 as 21.38% and 19.99%. No significant difference was found. The group with ethanol proportion as 0.8% reached less efficiencies, which are 16.40% and 14.71. The lowest efficiencies fell to less than 5% with the group that increased ethyl alcohol proportion to 1.0%.
Through the Potassium Dichromate closed reflux method, this researcher also found that ethanol reached the most effective utilization with proportion of 0.6% . In that proportion, B1 and B2 has utilized the ethanol up to 79%, which matched the effects of co-metabolism.
Keywords: 2-MIB, biodegradation, co-metabolism, Geosmin, musty/earthy orders
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