The Effect of Different Debye Lengths on Charged Ions Migration in Capillary Zone Electrophoresis

• Main Authors: Hsien-lin Tsai, 蔡憲琳
• Other Authors: Chen-I Hung
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/89079345622840408651

碩士 === 國立成功大學 === 機械工程學系碩博士班 === 97 === Capillary Zone Electrophoresis (CZE) is a kind of analysis method that develops rapidly in recent years. The effects of EDL cause different ions distribution density near the wall of CZE. Furthermore, the counter-ions will be pulled toward the microchannel wall of opposite charge and the co-ions will be pushed out of the EDL of the same charge. Therefore, the effect of different Debye lengths on charged ions migration in CZE will be investigated in this study. In order to get the more accurate result for circular-arc in the intersection of injection channel and separation channel, this study uses Finite Element Method (FEM) in the computation and employs the Particle-In-Cell (PIC) method to investigate the migration of negatively and positively charged ions in CZE chip. The results show that smaller Debye length has smaller effective zone and causes streamlines to expand widely, so many charged ions are pushed in separation channel. Because there is no cluster of negative-charged ions at arc-corner and smaller decreases of positive-charged ions during separation process, we can get more data output for analysis and more precise results.