Single Cell Impedance Measurement Using De-embedding Technique at Radio Frequency

碩士 === 國立成功大學 === 電機工程學系碩博士班 === 97 === When an electrical current with a low frequency is applied to a single cell, the pathological change of a cellular organelle that takes place inside the cell cannot be determined. To overcome the shortcomings of low frequency measurement, this paper combines a...

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Main Authors: Jen-yu Jao, 饒仁郁
Other Authors: Ling–Sheng Jang
Format: Others
Language:en_US
Published: 2009
Online Access:http://ndltd.ncl.edu.tw/handle/44571812471914589531
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spelling ndltd-TW-097NCKU54421712016-05-04T04:25:28Z http://ndltd.ncl.edu.tw/handle/44571812471914589531 Single Cell Impedance Measurement Using De-embedding Technique at Radio Frequency 應用去嵌入式技術於單細胞阻抗之射頻量測 Jen-yu Jao 饒仁郁 碩士 國立成功大學 電機工程學系碩博士班 97 When an electrical current with a low frequency is applied to a single cell, the pathological change of a cellular organelle that takes place inside the cell cannot be determined. To overcome the shortcomings of low frequency measurement, this paper combines a microfluidic device to capture single cells and a coplanar waveguide electrode inside a channel to measure the impedance of a single HeLa cell (human cervical epithelioid carcinoma) over the frequency range of 1 MHz to 1 GHz. The on-chip calibration technique is used to characterize the compound device. The technique improves the calibration accuracy by shifting the calibration reference plane to the de-embedding reference plane. This work presents a compound conductor backed coplanar waveguide (CBCPW) device which integrates two substrates for impedance analysis at the single-cell level. The outside part of the compound CBCPW is the FR4 substrate as the connection interface of a vector network analyzer (VNA) using a SubMiniature-A (SMA) connector. The inside part of the CBCPW is a glass substrate with electrodes to measure the impedance of a single cell. Various applications are demonstrated, including sodium chloride solutions with various concentrations, de-ionized (DI) water, alcohol, PBS, and a single HeLa cell. Equivalent circuit models of PBS and a single cell are used to explain the experimental impedance data. Ling–Sheng Jang 張凌昇 2009 學位論文 ; thesis 40 en_US
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language en_US
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description 碩士 === 國立成功大學 === 電機工程學系碩博士班 === 97 === When an electrical current with a low frequency is applied to a single cell, the pathological change of a cellular organelle that takes place inside the cell cannot be determined. To overcome the shortcomings of low frequency measurement, this paper combines a microfluidic device to capture single cells and a coplanar waveguide electrode inside a channel to measure the impedance of a single HeLa cell (human cervical epithelioid carcinoma) over the frequency range of 1 MHz to 1 GHz. The on-chip calibration technique is used to characterize the compound device. The technique improves the calibration accuracy by shifting the calibration reference plane to the de-embedding reference plane. This work presents a compound conductor backed coplanar waveguide (CBCPW) device which integrates two substrates for impedance analysis at the single-cell level. The outside part of the compound CBCPW is the FR4 substrate as the connection interface of a vector network analyzer (VNA) using a SubMiniature-A (SMA) connector. The inside part of the CBCPW is a glass substrate with electrodes to measure the impedance of a single cell. Various applications are demonstrated, including sodium chloride solutions with various concentrations, de-ionized (DI) water, alcohol, PBS, and a single HeLa cell. Equivalent circuit models of PBS and a single cell are used to explain the experimental impedance data.
author2 Ling–Sheng Jang
author_facet Ling–Sheng Jang
Jen-yu Jao
饒仁郁
author Jen-yu Jao
饒仁郁
spellingShingle Jen-yu Jao
饒仁郁
Single Cell Impedance Measurement Using De-embedding Technique at Radio Frequency
author_sort Jen-yu Jao
title Single Cell Impedance Measurement Using De-embedding Technique at Radio Frequency
title_short Single Cell Impedance Measurement Using De-embedding Technique at Radio Frequency
title_full Single Cell Impedance Measurement Using De-embedding Technique at Radio Frequency
title_fullStr Single Cell Impedance Measurement Using De-embedding Technique at Radio Frequency
title_full_unstemmed Single Cell Impedance Measurement Using De-embedding Technique at Radio Frequency
title_sort single cell impedance measurement using de-embedding technique at radio frequency
publishDate 2009
url http://ndltd.ncl.edu.tw/handle/44571812471914589531
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