A first astakine from Litopenaeus vannamei and its response to V. harveyi infection

• Main Authors: Ya-hui Chen, 陳雅惠
• Other Authors: Huey-lang Yang
• Format: Others
• Language: en_US
• Online Access: http://ndltd.ncl.edu.tw/handle/33834620157446756473

碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 97 === Astakine has been reported to involve in hematopoietic function that plays an important role in shrimp hematopoiesis, however, its molecular mechanism has not been clearly elucidated. An astakine was isolated by cloning from hemocytes of white shrimp Litopenaeus vannamei by 3' and 5' rapid amplification of cDNA ends (RACE) PCR. The cDNA of astakine consists of 1488 base pairs with a 372 base pairs open reading frame (ORF) able to code a 124 amino acids peptide. Nucleotide sequence analysis reveals that the nucleotide sequence of L. vannamei astakine has homology with that of Penaeus mondom and freshwater crayfish (Pacifastacus leniusculus) respectively. The putative protein domain contains a signal peptide start from amino acid 1 to 21, and a functional structure similar to prokineticin domain, locate from amino acid 22 to 107. The genomic DNA of astakine in ORF codes three exons and two introns. The gene expression of astakine from hemocytes and various tissues and the time course of astakine gene expression after sterile sea water or V. harveyi injection were analyzed with RT-PCR and sequence analysis. We suppose that expression of astakine may be regulated by alternative splicing and predicate that expression patterns of astakine show at last four types after injection.