| Summary: | 碩士 === 國立成功大學 === 化學系碩博士班 === 97 === Plumule rice coleoptiles with the length between 3 cm and 4 cm obtained
under hypoxia condition was re-incubated under aerobic condition by purging
air. It was found that there was a 28KDa protein, which concentration
increased with the increase of air purged time, in additional to the 16KDa
protein, which already exists in coleoptiles. Three methods, western blotting
analysis, cross-linking of the 28KDa protein with the bovine trypsin using
glutaraldehyde followed by Western blotting detection, and 2-D gel
electrophoresis analysis followed by LC/MSMS analysis and N-terminal
amino acid analysis, were employed to characterize this 28 kDa protein. The
results indicated that this 28 kDa protein is a protease inhibitor and it was part
of the protein of RBBI3-1 starting from the 37th amino acid. The extracts of
the coleoptiles, the plumule rice grains after incubation under aerobic and
hypoxia conditions, and the incubating medium used in both incubation
conditions were used to assay the antioxidant activity against DPPH. It was
found that plumule rice grains after incubation in the hypoxia condition
showed 73 % activity in the concentration of 5mg / m1.
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