Molecularly Imprinted Polymer Prepared by Sol-gel Process and Its Interior Transport Behavior

• Main Authors: Chun-Wei Hsu, 徐俊偉
• Other Authors: Ming-Chang Yang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/40011103953080481916

博士 === 國立成功大學 === 化學工程學系碩博士班 === 97 === In this study, a sol-gel method has been used to fabricate organic-inorganic hybrid molecularly imprinted polymers (MIPs) for application in polar solutions. Hydrophobic monomers were used as functional monomers to provide hydrophobic interaction in the polar solution. In the sol-gel system, the pH of sol solution was an important factor in fabricating the cholesterol imprinted polymers. The MIP had a larger pore volume with more non-specific binding of target molecule, when the formation of the polymer was catalyzed at a higher pH. For cholesterol-imprinted polymer, the largest imprinting-induced promotion of binding (IPB) value (13650 %) and lowest pore volume of the MIP was obtained with 0.01 M HCl in the sol-gel solution. Although the sol-gel procedure showed small amount of adsorption compared with acrylic polymer, it gave only a small amount of non-specific binding for both MIP and NIP when synthesized at low pH values. In addition to the ability of decreasing non-specific binding, the organic-inorganic hybrid MIPs showed facilitated transport toward template. Bisphenol A showed higher flux in the bisphenol A imprinted polymer (0.822 nmole/cm2.min) than in non-imprinted polymer (0.016 nmole/cm2.min). The phenomenon was believed due to the interaction between binding site and template and could be explained by the mechanism of facilitated transport in fixed-site carriers. The facilitated transport of template also could be observed in epinephrine imprinted polymer. The sensing current of epinephrine was higher in epinephrine imprinted polymer electrode (0.071 mA/mM) than in non-imprinted polymer electrode (0.008 mA/mM). The binding sites of epinephrine imprinted polymer played the major role in facilitated transport. The sensing current of epinephrine in epinephrine imprinted polymer electrode significantly decreased when 2-Phenethylamine was added into testing solution and occupied binding sites. In this study, MIPs showed high selectivity toward template. For cholesterol imprinted polymer, the amount of cholesterol adsorbed by cholesterol imprinted polymer was higher than cholecalciferol and other steroids in this study. Epinephrine imprinted polymer electrode showed good relative response toward epinephrine. The conformation, charge, and functional group of aromatic ring affected relative response of epinephrine imprinted polymer electrode toward epinephrine molecule.