| Summary: | 博士 === 國立成功大學 === 化學工程學系碩博士班 === 97 === Molecularly imprinted polymers (MIPs) are synthetic macromolecular structures with selective recognition, determined mainly by the shapes of their cavities, and the chemical functionality imbedded in the matrix. MIPs show potential for application in many fields, e.g. sensor design, chromatograpic separation and catalysis.
Bovine serum albumin (BSA) was selected as an imprinting target for this study as it is a direct mammalian analogue of its human counterpart. Albumin is a major transport protein in blood and as such the development of synthetic matrices able to recognize this protein have potential application in diagnostics.
Two polymer formulations were used to form imprints of BSA. Firstly, a BSA-MIP was prepared with a linear cross-linker, i.e. 3-Dimethylaminopropyl methacrylamide-Tetraethyleneglycol dimethacrylate (DMAPMA-TEGDMA), in the presence of 6.04％ water. This polymer on re-binding showed a high selectivity (99％) towards its template molecule, corresponding to 6.370 mg-BSA/ g-MIP. From the experimental data, the optimum mole ratio of DMAPMA to TEGDMA, was 1：8, the optimal polymerization time was 60 hr with the synthesis being carried out at 38℃.
In the second approach, TEGDMA was replaced with Pentaerythritol tetraacrylate (PETTA), which is a non-linear cross-linking agent with a more rigid structure than TEGDMA therefore allowing less conformational freedom during MIP formation. From the experimental data, the optimum synthesis conditions of this system were determined as 48 hr polymerization at 38℃ and the mole ratio of DMAPMA to PETTA was 1：11. This MIP, formulated with DMAPMA and PETTA, showed a 93.56％ selectivity to BSA with a similar adsorption ability as the DMAPMA-TEGDMA co-polymer. Based on this result, we may deduce that the linear cross-linker is better optimised for preparation of the BSA imprinted polymer.
Application of the DMAPMA-TEGDMA MIP in different settings, such as in: sensors or thin film fabrication, gave good data. The MIP-QCM sensor showed excellent selectivity towards BSA. Synthesis of MIPs using micro-contact imprinting also showed good results. Also included are SEM and AFM images of MIP thin films made by micro-contact imprinting showing their surface morphology.
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