| Summary: | 碩士 === 國立成功大學 === 口腔醫學研究所 === 97 === Titanium and its alloys are widely used in biomaterials because of their good biocompatibility. When titanium was contact with the air or fluids, the high activities of Ti would drive the surface to forming a several nm thickness oxide layer. Osteoblast cells would lead to the pathway of proliferation and differentiation by adhered to the oxide layer, forming active bindings. The process is also called osseointegration. It had found that the flat Ti surface limited osteoblast cells to growth because of the low surface area. To prove the problem, surface modification is used to increase cell adaptation. The aim of the study is to use the self- assembly monolayers (SAMs) modified TiO2 nanotubes array surface to observed the HOS growth behaviors by modulated the nanotube-forming factors and the different functional end group SAM.
The key factor of the TiO2 nanotube formation is the concentration of HF and F . The acid dissociation constant of HF is not high enough to forming the TiO2 nanotubes in the anodic oxidation process with lower [F-]. To increase the [F-], NaOH, which can dissociate HF, and boarding Pt cathode electrode, are both the good ideas. The SAMs were formed by immersion. By contact angle test, the use of RF O2 plasma treatment has the ability to enhance the grafting of SAMs with higher contact angle. In the in vitro test, surface with nanotubes was good for cell to adhesion except the flat surface. To understand cell growth behavior by the surface of TiO2 nanotubes with SAM, cell proliferation and differentiation test could accurately proved.
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