Isolation and Identification of Bacteria and Fungi from Canine Dermatitis Patients

• Main Authors: Caryl Riconalla, 李凱洛
• Other Authors: Cheng-Hung Lai
• Format: Others
• Language: en_US
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/76782602625760322159

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 97 === Dermatitis is a commonly encountered disease in dogs and its treatment often involves antimicrobial therapy. It is caused by a wide range of factors including bacterial, fungal, allergic, parasitic and atopic etiologic agents. Moreover, many studies have reported the frequent isolation of multidrug-resistant strains from canine dermatitis patients and failure in treatment causes problems in small animal practice. Thus, the purpose of this study is to identify the common bacterial and fungal isolates from canine dermatitis patients and to assess the frequency of their resistance to commonly used antibiotics. There were 26 bacterial isolates obtained from various canine dermatitis patients from NCHU Veterinary Teaching Hospital. The isolates were identified using Biolog® microbial ID system, and were subjected to antibiotic sensitivity tests. Among the bacterial isolates S. intermedius B (26.92%) is the most abundant, other isolates were S. aureus ss aureus (23.08%), Pseudomonas aeruginosa (11.54%), other Staphylococci spp. (19.23%), and other bacteria (19.23%). Eighteen out of the 26 isolates showed resistance to at least one or more type of antibiotic. Furthermore, we were able to identify 15 fungal isolates belonging to 11 different species, namely Madurella grisea (2/15), Aspergillus fumigatus (2/15), Aspergillus sp. (2/15), Trichophyton tonsurans (2/15), Microsporum gallinae (1/15), Microsporum nanum (1/15), Penicillium sp. (1/15), Streptomyces sp. (1/15), Malbranchea sp. (1/15), Candida guillermondii (1/15), and Cladosporium sp. (1/15). In this study, we have also shown that the use of universal primers ITS1-ITS4 along with RFLP using HaeIII yielded specific banding patterns and thus could serve as a tool for the differentiation of various fungal species causing dermatitis.