Comparing the Effects of Adipose Tissue-derived Stem Cells (ADSCs) and Bone Marrow-derived Mesenchymal Stem Cells (BMSCs) on the Growth and Secretory Activity of Rabbit Nucleus Pulposus Cells

• Main Authors: Herng Kuo, 郭衡
• Other Authors: 黃勇三
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/11873853600000697552

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 97 === Intervertebral disc degeneration is due to a decrease of proteoglycan which impairs the ability of nucleus pulposus to imbibe water and reduces the normal load bearing ability of the disc. The studies of disc degeneration treatment had proved that coculture with bone marrow-derived mesenchymal stem cells (BMSCs) raised efficacy in activating nucleus pulposus cells (NPCs), including upregulation of cell proliferation and extracellular matrix production. Adipose tissue-derived stem cells (ADSCs) arise from mesenchyma, unlike BMSCs, are easier to obtain. Many researchers demonstrated that the BMSCs and ADSCs had an equal potential to differentiate into cells and tissues of mesodermal origin. In this study, histochemistry and ELISA were used to compare the effects of cell proliferation, and extracellular matrix production on NPCs when cocultured with BMSCs and ADSCs. Results indicated that both BMSCs and ADSCs raised efficacy in cell proliferation of NPCs. Furthermore, BMSCs could promote extracellular matrix production of NPCs, but the effect was not conspicuous when NPCs were cocultured with ADSCs. In conclusion, we suggest that BMSCs are still more effective on increasing the proliferative and matrix synthesis capacity of NPCs.