A capillary electrophoresis chip intergrated with the contactless conductimetric detector for the detection of amino acid of tea

• Main Authors: Yen-Chung Chen, 陳彥鐘
• Other Authors: 吳靖宙
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/98353588018807560878

碩士 === 國立中興大學 === 生物產業機電工程學系所 === 97 === In addition to being a daily beverage, tea contains abundant functional compounds, which are beneficial for human health. It is important to detect the quality and safety of tea. Therefore, a miniature capillary electrophoresis (CE) chip integrated with a conductimetric detector for the detection of theanine and γ-aminobutyric acid (GABA) has been developed. The CE chip uses an indium tin oxide (ITO) glass as the electrode substrate to fabricate ITO microelectrodes by using microfabrication techniques, and the insulator of ITO electrodes and sidewalls of CE channel was made of negative photoresist, SU8. Moreover, the ITO electrode substrate was bonded with another glass substrate using hot-bonding and medium-bonding techniques. Owning to the pI value of GABA and theanine respectively corresponding to 7.29 and 5.65, they present positive charges in the 10 mM acetic acid (pH 2.65) running buffer. The linear range, sensitivity and detection limit of GABA measured by planar two-electrode contactless conductimetry was 0.1 mM－5 mM, 101.9