| Summary: | 碩士 === 國立中興大學 === 園藝學系所 === 97 === The clonal plants of blossomed Paphiopedilum (Paph. delenatii × Paph. armeniacum) hybrids propagated by division were used as materials for initial culture. It succeeded to develop lateral shoots from the stem explants.When the material drought for two weeks before culture, the survival rate increased. To sterile by 10% NaOCl with 0.1% Tween 20 for 9 minutes resulted in higher survival rate. Also, material with five leaves resulted in higher survival rate. Moreover, explants cultured on hormone free medium enhanced the survival rate to 66.7%.
When the concentration of CuSO4 ．5H2O in MS medium raised to 0.75 mg/l, explant sprouted 1 shoot. New shoots developed more shoots, but shorter roots from explants in liquid medium than that in solid medium. In the case of decreasing 1/3 the boron concentration of MS medium promoted shoots earlier.
When yeast extract as nitrogen source in the MS medium without NH4NO3 and KNO3, proliferated 3.67 shoots. Although explants on the medium containing higher concentration of TDZ (16 mg/l) proliferated more shoots than less one (8 mg/l ). The proliferated shoots were fewer than on medium containing BA 50 mg/l. In addition, explants excised leaves enhance new shoots production and inhibited rooting.
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