The Mutation of Kalanchoe via Sodium Azide in vitro

• Main Authors: Sujittra Tejakhod, 蘇姫泰
• Other Authors: Chien-Young Chu
• Format: Others
• Language: en_US
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/66068951263644203784

碩士 === 國立中興大學 === 園藝學系所 === 97 === The application of induced mutation in vitro by using sodium azide (SA) consisted of culturing explant in SA medium, and soaking explant in mutagen solution treatment. Floret bud from 2nd level of Kalanchoë blossfeldiana ‘Beacon’ and ‘Sunrise cyme inflorescence were used as explant that culture media was used ½ MS containing BA 1mgl-1 combined NAA 0.5mgl-1 as presented as the most proliferated shoots medium in pretreatment. Whereas, in leaf experiment were used marginal leaf explant of ‘Beacon’ cultured on basal medium consisted of BA 1 mgl-1 combined 0.5 mgl-1 NAA, and leaf vein explant of ‘Sunrise’ cultured in media supplemented with 2 mgl-1 BA and 0.5 mgl-1 NAA, combined with SA treatment to induced mutation. Plantlets from control groups of ‘Beacon’ and ‘Sunrise’ were found up to 90% of survival, whereas both of application of culturing explants in SA medium or soaking in this mutagen solution had lower shoot regeneration frequency. The mutation rate via flower buds or leaf explant of ‘Beacon’ was 77.8 or 53.1%, respectively; whereas the rate of mutation cv. Sunrise was 53.1% from flower buds as well as 41% from leaf explants. The results of this experiment proved that SA regenerated plants showed greater variation in the leaf phenotype compared to the plants regenerated from the control. Furthermore, mutants of floral change were accompanied with leaf change. The original of ‘Beacon’ cultivar had flower color as bright orange, but mutant plants were found ‘lighter orange’ and ‘yellow’. In addition, the petal colour of ‘Sunrise’ mutant changed from bright red into ‘deep red’, ‘dull red’, or ‘orange’. Early flowering was one of induced mutation in physiological trait that obtained from both of ‘Beacon’ and ‘Sunrise’ mutants.