| Summary: | 碩士 === 國立中興大學 === 動物科學系所 === 97 === This study was conducted to investigate the effects of dietary fat sources and selenium levels on lipid metabolism of White Roman geese by two experiments. In experiment I, 20 White Roman breeding geese with 3th non-laying period were used. In experiment Ⅱ, 20 White Roman breeding geese with 4th laying period were used. Geese of two experiments were randomly divided into 4 treatments and kept in windowless environmental-controlled house with wet pad cooling system. The lighting program was 16L:8D atternating with 9L:15D in both experiments. The room temperature was maintained at 26~30℃ during experimental period. Feed and water were supplied ad libutum for 4-week experiment period. Were fed on two different sources of dietary fat, i.e., 3.5% Fish oil (FO) or 3.5% Lard (LO) and two levels of selenium, i.e, 0 ppm or 0.15 ppm. At the end of the experiment, liver, blood and abdominal fat pad were sampled. The concentrations of triglyceride, total cholesterol and total lipid in the serum and liver, estradiol and progesterone in the serum, hepatic lipogenic related enzyme activities and fatty acid profiles were analysed. The results of experiment Ⅰindicated that the different dietary fat didn’t affect final body weight, abdominal fat and relative abdominal fat weights(P > 0.05). Liver and relative liver weights and the concentrations of total cholesterol, total lipid and estradiol in the plasma in FO group were significant lower (P < 0.05) than those in LO group. However fat source didn’t affect the concentrations of triglyceride, high density lipoprotein and low density lipoprotein cholesterol and progesterone in plasma. The concentration of total cholesterol in the liver significantly higher in FO group than that in LO group (P < 0.05). The activities of hepatic lipogenic related enzymes including fatty acid synthetase (FAS), NADP-malate dehydrogenase (NADP-MDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) weren’t significantly different among treatments (P > 0.05). Abdominal fat and liver polyunsaturated fatty acids was significantly higher in FO group than those in LO group, but saturated fatty acids was significantly lower than LO (P < 0.05). Diets supplemented with different selenium levels weren’t significantly difference in the final body weight, liver and liver relative weights, abdominal and abdominal relative weights, plasma and liver lipid concentrations among treatments (P > 0.05). In the activities of hepatic lipogenic related enzymes, FAS was significantly higher in selenium group than those in without selenium group (P < 0.05); NADP-MDH and G-6-PDH weren’t significantly difference among treatments (P > 0.05). The results of experiment Ⅱ indicated that the different sources of dietary fat didn’t affect final body weight, liver and liver relative weights, abdominal and abdominal relative weights, plasma and liver lipid concentrations(P > 0.05). The activities of hepatic lipogenic related enzymes including FAS and NADP-MDH weren’t significantly different among treatments (P > 0.05). The LO group had significantly higher G-6-PDH activity than FO group (P < 0.05). In Fatty acid profiles, abdominal fat and liver polyunsaturated fatty acids were significantly higher in FO group than those in LO group (P < 0.05). Diets supplemented with different selenium levels weren’t significantly difference in the final body weight, liver and liver relative weights, abdominal and abdominal relative weights. hepatic lipogenic related enzyme, plasma and liver lipid concentration among treatments (P > 0.05). In the liver fatty acid profiles, birds fed selenium supplemented group had significantly higher saturated fatty acids and polyunsaturated fatty acids than those fed without selenium supplemented group (P < 0.05).
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