Comparisons of H-FABP, RN, CRC, CA3, ESR and PRLR Genotypes among Different Breeds of Pigs

• Main Authors: Pratthana Jamwan, 趙蓓娜
• Other Authors: Mu-Chiou Huang
• Format: Others
• Language: en_US
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/48638999010206521864

碩士 === 國立中興大學 === 動物科學系所 === 97 === The aim of this study was to comparisons of H-FABP, RN, CRC, CA3, ESR, and PRLR genotypes in Thai and Taiwan pigs using minisequencing and PCR-RFLP. The H-FABP gene is a candidate gene for intramuscular fat (IMF) content. The highest frequencies of HH-6 (0.646) in Taiwan Duroc, HL-5 (0.200) in Thai Pietrain, HL-4 (0.694) in Taiwan Landrace, HL-3 (0.650) in Thai 2x dam line, HL-2 (0.400) in Thai Large White, HL-1 frequency (0.380) in Thai Black pig, and LL-0 (0.161) in Taiwan Black pig were found. The RN gene is a candidate gene for acid meat. The phonotype of rn+/rn+ is normal. All genotypes of RN gene in tested animals were rn+/rn+ (1.000) other than Thai Landrace (0.950), Taiwan Duroc (0.994) and Taiwan Black pig (0.935). The CRC gene is candidate gene for PSE and PSS, all AA type (normal) (1.000) was observed in Large White, Landrace and Duroc pigs in both of Thai and Taiwan. The highest frequency of AB type (0.579) was found in 2x sire line of Thai pigs. There was still minor B allele in Pietrain, 2x dam line (Thai) and Black pigs (both of Thai and Taiwan). The CA3 (C/T) gene is a candidate gene for lean cuts (LC) and visible intermuscular fat (VIF). The highest frequencies of CC (0.900) in Thai Pietrain, CT (0.650) in Thai Landrace and TT (0.800) in Thai Large white were found. The CA3 (A/G) is a candidate gene for percentage of ham. All tested animals genotypes are AA (1.000). The ESR is a candidate gene for reproduction. The highest frquencies of AA (1.000) found in Taiwan Duroc, AB (0.480) in 2x dam line, and BB (0.650) in Thai Large White. The PRLR gene also is a candidate gene for reproduction. The highest frequencies of AA (0.624) in Taiwan Black pig, AB (0.800) in Thai Large White and BB (1.000) in Thai Pietrain were found. These results might provide the information for genetic improvement in pig breeding.