| Summary: | 碩士 === 國立中興大學 === 物理學系所 === 97 === We assemble the in vivo imaging system successfully. Our system has minimizes noise, and maximizes sensitivity CCD, can quantitate outcomes of in vivo imaging of bioluminescent or fluorescent reporters. The system modulate view field by computer.
The in vivo imaging system allows researchers to perform in vivo biophotonic imaging that uses real-time imaging to monitor and record cellular and genetic activity within a living organism by using fluorescent and/or bioluminescent reporters. Principle is use CCD detect light signal form internal luminescent dye in animal body.
We do two improvement on our system: (1)Optimize location of emission filter: we locate emission filter behind imaging lens without secondary imaging lens, this design allow our system use smaller emission filter to save cost and easy to modulate lens stage. (2)Uniform excitation light: we uniform light by integrating rod, do not lose light intensity, also improve imaging lens to expand uniform light into area 20x20 (cm2) in length 40 (cm), and the Uniformity is more than 90%. Uniform light help researchers to analyze quantitative data.
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