| Summary: | 碩士 === 國立中興大學 === 昆蟲學系所 === 97 === This study is to investigate the virus susceptibility of Culex quinquefasciatus, Aedes albopictus, and Armigeres subalbatus to Japanese encephalitis virus. The infection rate of Cx. quinquefasciatus, Ae. albopictus, and Ar. subalbatus to JEV at 14 days post infection by oral feeding were 16.67%, 15.28% and 11.11% at 20°C; 34.24%, 37.50% and 15.66% at 25°C; 76.67%, 66.67% and 52.63% at 30°C, respectively. There were significant differences in the susceptibility of JEV among these three mosquitoes. Tissue tropism of JEV showed that head and midgut were the highest infected tissues, followed by salivary glands, while the reproductive tissue was rarely infected by JEV. Generally, the virus infection rate is higher when the temperature increases from 20°C to 30°C, and also increases with the days after post infection. When these mosquitoes were infected by JEV via intrathoracic injection, the oral transmission rate of Cx. quinquefasciatus, Ae. albopictus, and Ar. subalbatus to JEV were 96%, 76% and 72%, respectively. High oral transmission rate suggests these mosquitoes can effectively transmit JEV when it was infected with JEV. No vertical transmission of JEV in the three mosquitoes had been found. Whether Cx. quinquefasciatus, Ae. albopictus, and Ar. subalbatus are the vectors of JEV should be further studied.
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